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The main objective of this handbook is to guide the development of investable NbS propositions and bankable NbS projects for both public and private investors through different modes of governance, funding and implementation arrangements. It offers operational advice and project preparation guidelines, including hands-on formats that project proponents can use continuously and iteratively to develop the entire business case of NbS, enabling them to go from the idea stage towards an investment proposal or investment project stage that can be effectively assessed by potential investors. This makes the handbook a comprehensive and first of a kind document as it adapts the process of developing a business case to the specificities of NbS investments for water security.